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TopoView

TopoView is a Topology Viewer for alpha-helical transmembrane proteins

Researcher of this topic: Nadine Schneider

Residue-based diagrams of proteins, also called 'snake plots', are often employed for representing alpha-helical transmembrane  proteins as long as their three-dimensional structures are not available. This 2D representation allows visualizing the helical portions of the protein sequence and, in the case of transmembrane proteins, the topology (i.e. the orientation of the N-terminus with regard to the lipid bilayer). The tool TopoView is such a topology viewer for alpha-helical TM proteins. Given an amino acid sequence and the borders of the TM segments, it produces a high quality 2D representation of this protein sequence. As special features, one may visualize user-specified structural features such as interface helices, disrupted transmembrane helices or non contacting consecutive transmembrane helices.

TopoView image1

Fig1: Topology image of the Cytochrome bc1 complex (1BCC)where the non contacting consecutive helices are highlighted in green,the globular domains are illustrated as blue spheres, and helices 10 and 11 represent a long 'broken' transmembrane helix as two short helices lying above each other.

Implementation: The tool TopoView was implemented in Python(http://www.python.org/) as Python is widespread, platform independent and efficient compilers are freely available. Also, a graphical user interface was integrated. The high-quality images produced by TopoView are rendered by means of the tool POV-Ray (http://www.povray.org/)(Persistance of Vision Raytracer). POV-Ray is another platform independent open source project that creates stunning three-dimensional graphics. Therefore, current Python and POV-Ray releases are prerequisites for utilizing TopoView.

TopoView image2

Fig 2: Another example of a topology image of the Cytochrome bc1 Complex (1BCC). Here, only the fasta sequence was given as input and a prediction of the transmembrane segments made by the prediction server TMHMM v2.0 (www.cbs.dtu.dk/services/TMHMM/).

The tool TopoView can be downloaded as a stand-alone program for linux systems:

download TopoView

After downloading the file, unzip the file, go to the folder 'source' and then launch the application by executing:

python TopoView.py

The documentation (user's manual) is located in the folder 'docs' of TopoView

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